Composite

Part:BBa_K4724084:Design

Designed by: Xinyao Li   Group: iGEM23_NJTech-China-A   (2023-10-09)


ompA-LSPETase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 895
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 151
    Illegal AgeI site found at 238
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insert the signal peptide OmpA gene sequence into the vector pET-22b (+) - LSPET-12, and construct a recombinant plasmid using the Gibson assembly method to promote the soluble expression of the original target fragment LSPET protein.


Source

An enzyme identified in the laboratory of our research group that can identify PET

References